{"id":9314,"date":"2024-03-25T07:00:21","date_gmt":"2024-03-24T22:00:21","guid":{"rendered":"https:\/\/clarivate.com\/ja\/?p=9314"},"modified":"2024-03-25T07:00:21","modified_gmt":"2024-03-24T22:00:21","slug":"tips-for-improving-reading-and-writing-abstracts-of-english-paper-part1-jpn","status":"publish","type":"post","link":"https:\/\/clarivate.com\/academia-government\/ja\/blog\/tips-for-improving-reading-and-writing-abstracts-of-english-paper-part1-jpn\/","title":{"rendered":"\u82f1\u8a9e\u8ad6\u6587\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u306e\u8aad\u307f\u66f8\u304d\u4e0a\u9054\u306e\u30b3\u30c4\uff1a\u7b2c1\u56de\u300c\u82f1\u8a9e\u8ad6\u6587\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u306e\u7d20\u65e9\u3044\u8aad\u307f\u65b9\u300d"},"content":{"rendered":"

2024\u5e74\u6625\u3001\u56fd\u969b\u30b8\u30e3\u30fc\u30ca\u30eb\u306b\u63b2\u8f09\u3055\u308c\u305f\u82f1\u8a9e\u8ad6\u6587\u3092\u8aad\u3093\u3067\u5148\u884c\u7814\u7a76\u3092\u6df1\u3081\u305f\u3044\u65b9\u3005\u3001\u82f1\u8a9e\u8ad6\u6587\u3092\u57f7\u7b46\u3057\u3066\u7814\u7a76\u5185\u5bb9\u3092\u4e16\u754c\u306b\u767a\u8868\u3059\u308b\u3053\u3068\u3092\u671b\u307e\u308c\u3066\u3044\u308b\u7814\u7a76\u8005\u306e\u65b9\u3005\u3001\u4f55\u304b\u3089\u306f\u3058\u3081\u308c\u3070\u3088\u3044\u304b\u3068\u8ff7\u3063\u3066\u304a\u3089\u308c\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u305d\u306e\u3088\u3046\u306a\u5834\u5408\u306b\u306f\u3001\u307e\u305a\u306f1\u65e51\u4ef6\u3001\u82f1\u8a9e\u8ad6\u6587\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u3092\u8aad\u3080\u3053\u3068\u304b\u3089\u306f\u3058\u3081\u3066\u3044\u305f\u3060\u304f\u306e\u304c\u52b9\u679c\u7684\u3067\u3059\u3002\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u306f100\u30ef\u30fc\u30c9\u7a0b\u5ea6\u304b\u3089300\u30ef\u30fc\u30c9\u7a0b\u5ea6\u306e\u7bc4\u56f2\u30671\u3064\u306e\u72ec\u7acb\u3057\u305f\u30b9\u30c8\u30fc\u30ea\u30fc\u304c\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u8aad\u307f\u3084\u3059\u304f\u3001\u6280\u8853\u82f1\u8a9e\u306e\u5b66\u7fd2\u306b\u3082\u6700\u9069\u3067\u3059\u3002\u8208\u5473\u3042\u308b\u5206\u91ce\u304b\u3089\u8aad\u307f\u306f\u3058\u3081\u3001\u6642\u306b\u306f\u624b\u5f53\u305f\u308a\u6b21\u7b2c\u3001\u8aad\u307f\u9032\u3081\u3066\u3044\u304f\u306e\u3082\u3088\u3044\u3067\u3057\u3087\u3046\u3002<\/p>\n

\u539f\u8457\u8ad6\u6587\u306e\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u306b\u306f\u3001\u6b21\u306e\u3088\u3046\u306a\u5185\u5bb9\u304c\u8a18\u8f09\u3055\u308c\u308b\u306e\u304c\u4e00\u822c\u7684\u3067\u3059\u3002<\/p>\n

    \n
  1. \u4e3b\u984c\u3084\u554f\u984c\u306e\u63d0\u793a\uff08\u8ad6\u6587\u672c\u6587\u306e\u300c\u5e8f\u8ad6\u300d\u306b\u76f8\u5f53\uff09<\/li>\n
  2. \u5b9f\u969b\u306b\u4f55\u3092\u884c\u3063\u305f\u304b\u306e\u8aac\u660e\uff08\u8ad6\u6587\u672c\u6587\u306e\u300c\u65b9\u6cd5\u300d\u306b\u76f8\u5f53\uff09<\/li>\n
  3. \u4e3b\u8981\u306a\u7d50\u679c\u3084\u793a\u5506\u306e\u63d0\u793a\uff08\u8ad6\u6587\u672c\u6587\u306e\u300c\u7d50\u679c\u3068\u8003\u5bdf\u300d\u306b\u76f8\u5f53\uff09<\/li>\n<\/ol>\n

    \u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u5185\u3067\u306e1\uff5e3\u306e\u5206\u91cf\u306e\u914d\u5206\u306f\u3001\u8ad6\u6587\u306e\u5185\u5bb9\u3084\u8457\u8005\u306e\u66f8\u304d\u65b9\u306b\u3088\u3063\u3066\u3055\u307e\u3056\u307e\u3067\u3059\u3002\u30af\u30e9\u30ea\u30d9\u30a4\u30c8\u793e\u306e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u300cWeb of Science\u2122\u300d\u3092\u4f7f\u3063\u3066\u3001\u82f1\u8a9e\u8ad6\u6587\u3092\u62bd\u51fa\u3057\u3066\u8aad\u3093\u3067\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n

    \u4e00\u4f8b\u3068\u3057\u3066\u3001\u300cmRNA\u533b\u85ac\u300d\uff08\u4ee4\u548c6\u5e74\u306e\u300c\u7279\u8a31\u51fa\u9858\u6280\u8853\u52d5\u5411\u8abf\u67fb\u300d\u306e\u5316\u5b66\u5206\u91ce\u30c6\u30fc\u30de\uff09\u3092\u30c8\u30d4\u30c3\u30af\u306b\u6307\u5b9a\u3057\u3066\u3001\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u3092\u62bd\u51fa\u3057\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n

    \u30bf\u30a4\u30c8\u30eb\uff1aSelective hydrophobic interaction chromatography for high purity of supercoiled DNA plasmids
    \nBIOTECHNOLOGY AND BIOENGINEERING, Feb 14, 2024\uff08\u65e9\u671f\u516c\u958b\uff09<\/p>\n

    \u307e\u305a\u306f\u30bf\u30a4\u30c8\u30eb\u3067\u3059\u3002\u30c6\u30fc\u30de\u304c\u300cselective hydrophobic interaction chromatography\uff08\u9078\u629e\u7684\u758e\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\uff09\u300d\u3067\u3001\u7528\u9014\u306f\u300cfor high purity of supercoiled DNA plasmids\uff08\u8d85\u87ba\u65cbDNA\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u9ad8\u7d14\u5ea6\u5316\uff09\u300d\u3067\u3059\u3002\u82f1\u8a9e\u306f\u540d\u8a5e\u306e\u56fa\u307e\u308a\u3054\u3068\u306b\u610f\u5473\u3092\u7406\u89e3\u3067\u304d\u308b\u52b9\u679c\u7684\u306a\u8a00\u8449\u3067\u3059\u306e\u3067\u3001\u524d\u7f6e\u8a5e\u306e\u524d\u3067\u533a\u5207\u308b\u306e\u304c\u5f97\u7b56\u3067\u3059\u3002\u8aad\u307f\u624b\u306e\u982d\u306e\u4e2d\u3067\u3001\u6b21\u306e\u3088\u3046\u306b\u51e6\u7406\u3059\u308b\u306e\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002<\/p>\n

    \uff1c\u8aad\u307f\u65b9\uff1e<\/p>\n\n\n\n\n\n\n\n\n
    Selective<\/td>\n\u9078\u629e\u7684\u306a<\/td>\n<\/tr>\n
    hydrophobic interaction chromatography<\/td>\n\u758e\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc<\/td>\n<\/tr>\n
    for<\/td>\n\u305d\u306e\u7528\u9014\u306f<\/td>\n<\/tr>\n
    high purity<\/td>\n\u9ad8\u7d14\u5ea6<\/td>\n<\/tr>\n
    of<\/td>\n\u4f55\u3092<\/td>\n<\/tr>\n
    supercoiled DNA plasmids<\/td>\n\u8d85\u87ba\u65cbDNA\u30d7\u30e9\u30b9\u30df\u30c9<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

    \u96e3\u306a\u304f\u30bf\u30a4\u30c8\u30eb\u3092\u7406\u89e3\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u305f\u3067\u3057\u3087\u3046\u304b\u3002\u9577\u304f\u3066\u96e3\u3057\u3044\u3068\u611f\u3058\u308b\u30d5\u30ec\u30fc\u30ba\u306f\u3001\u5f62\u5bb9\u8a5e\u5358\u4f53\uff08\u4f8b\uff1aselective\uff09\u3067\u533a\u5207\u308b\u306e\u3082\u304a\u3059\u3059\u3081\u3067\u3059\u3002<\/p>\n

    \u3055\u3066\u3001\u6b21\u306b\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u672c\u6587\u3092\u8aad\u307f\u307e\u3059\u3002\u307e\u305a\u306f\u5168\u4f53\u3092\u5927\u307e\u304b\u306b\u8aad\u307f\u3001\u3069\u3053\u306b\u4f55\u304c\u66f8\u3044\u3066\u3042\u308b\u304b\u3092\u628a\u63e1\u3057\u307e\u3057\u3087\u3046\u3002\u5148\u306b\u3042\u3052\u305f\u30011. \u4e3b\u984c\u3084\u554f\u984c\u306e\u63d0\u793a\uff08\u8ad6\u6587\u672c\u6587\u306e\u300c\u5e8f\u8ad6\u300d\u306b\u76f8\u5f53\uff09\u30012. \u5b9f\u969b\u306b\u4f55\u3092\u884c\u3063\u305f\u304b\u306e\u8aac\u660e\uff08\u8ad6\u6587\u672c\u6587\u306e\u300c\u65b9\u6cd5\u300d\u306b\u76f8\u5f53\uff09\u30013. \u4e3b\u8981\u306a\u7d50\u679c\u3084\u793a\u5506\u306e\u63d0\u793a\uff08\u8ad6\u6587\u672c\u6587\u306e\u300c\u7d50\u679c\u3068\u8003\u5bdf\u300d\u306b\u76f8\u5f53\uff09\u3092\u63a2\u3059\u305f\u3081\u306b\u3001\u5404\u6587\u306e\u300c\u4e3b\u8a9e\u300d\u3068\u300c\u52d5\u8a5e\u300d\u3092\u5927\u307e\u304b\u306b\u78ba\u8a8d\u3057\u306a\u304c\u3089\u8aad\u307f\u9032\u3081\u307e\u3059\u3002<\/p>\n

    \u5192\u982d\u3067\u306f\u3001\u4e3b\u984c\u30fb\u554f\u984c\u304c\u63d0\u793a\u3055\u308c\u307e\u3059\u3002\u6b21\u306b\u9375\u3068\u306a\u308b\u306e\u306f\u30015\u6587\u76ee\u306e\u4e3b\u8a9eWe\u3067\u3001\u7b46\u8005\u304c\u884c\u3063\u305f\u5185\u5bb9\u3078\u3068\u60c5\u5831\u304c\u79fb\u884c\u3057\u305f\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u307e\u305f\u5f8c\u534a\uff089\u6587\u76ee\uff09\u306b\u3082\u4e00\u4eba\u79f0\u306e\u4e3b\u8a9eWe\u304c\u767b\u5834\u3057\u3001\u7814\u7a76\u3092\u901a\u3058\u3066\u4f55\u304c\u308f\u304b\u3063\u305f\u304b\u304c\u63d0\u793a\u3055\u308c\u307e\u3059\u3002<\/p>\n

    \n
    \n

    1. \u4e3b\u984c\u3084\u554f\u984c\u306e\u63d0\u793a\uff081\u6587\u76ee\uff09High purity of plasmid DNA (pDNA), particularly in supercoiled isoform (SC), is used for various biopharmaceutical applications, such as a transfecting agent for production of gene therapy viral vectors, for pDNA vaccines, or as a precursor for linearized form that serves as a template for mRNA synthesis. \uff082\u6587\u76ee\uff09In clinical manufacturing, pDNA is commonly extracted from Escherichia coli cells with alkaline lysis followed by anion exchange chromatography or tangential flow filtration as a capture step for pDNA. \uff083\u6587\u76ee\uff09Both methods remove a high degree of host cell contaminants but are unable to generically discriminate between SC and open-circular (OC) pDNA isoforms, as well as other DNA impurities, such as genomic DNA (gDNA). \uff084\u6587\u76ee\uff09Hydrophobic interaction chromatography (HIC) is commonly used as polishing purification for pDNA. 2. \u5b9f\u969b\u306b\u4f55\u3092\u884c\u3063\u305f\u304b\u306e\u8aac\u660e\uff085\u6587\u76ee\uff09We developed HIC-based polishing purification methodology that is highly selective for enrichment of SC pDNA. \uff086\u6587\u76ee\uff09It is generic with respect to plasmid size, scalable, and GMP compatible. \uff087\u6587\u76ee\uff09The technique uses ammonium sulfate, a kosmotropic salt, at a concentration selective for SC pDNA binding to a butyl monolith column, while OC pDNA and gDNA are removed in flow-through. \uff088\u6587\u76ee\uff09The approach is validated on multiple adeno-associated virus- and mRNA-encoding plasmids ranging from 3 to 12 kbp. 3. \u4e3b\u8981\u306a\u7d50\u679c\u3084\u793a\u5506\u306e\u63d0\u793a\uff089\u6587\u76ee\uff09We show good scalability to at least 300 mg of >95% SC pDNA, thus paving the way to increase the quality of genomic medicines that utilize pDNA as a key raw material.<\/p>\n<\/div>\n

     <\/p>\n

    \u3053\u306e\u3088\u3046\u306b\u3001\u3069\u3053\u306b\u4f55\u304c\u66f8\u3044\u3066\u3042\u308b\u304b\u3092\u5927\u307e\u304b\u306b\u628a\u63e1\u3057\u305f\u4e0a\u3067\u3001\u6b21\u306b\u3001\u8aad\u307f\u305f\u3044\u90e8\u5206\u3092\u91cd\u70b9\u7684\u306b\u78ba\u8a8d\u3057\u307e\u3059\u3002\u300c\u4f55\u3092\u89e3\u6c7a\u3057\u305f\u3044\u8ad6\u6587\u304b\u300d\u3092\u77e5\u308b\u305f\u3081\u306b\u306f\u5192\u982d\u306e1\u6587\u76ee\uff5e4\u6587\u76ee\u3001\u300c\u672c\u8ad6\u6587\u3067\u4f55\u304c\u884c\u308f\u308c\u305f\u306e\u304b\u300d\u3092\u77e5\u308a\u305f\u3044\u5834\u5408\u306b\u306f5\u6587\u76ee\uff5e8\u6587\u76ee\u3001\u300c\u4e3b\u8981\u306a\u7d50\u679c\u3084\u4eca\u5f8c\u306e\u898b\u89e3\u300d\u306b\u3064\u3044\u3066\u77e5\u308a\u305f\u3044\u5834\u5408\u306b\u306f\u3001\u6700\u5f8c\u306e1\u6587\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002<\/p>\n

    \u65e9\u901f\u3001\u4e0a\u8a181\uff5e3\u306e\u5404\u90e8\u5206\u304b\u3089\u629c\u7c8b\u3057\u305f\u6587\u7ae0\u3092\u78ba\u8a8d\u3057\u3001\u60c5\u5831\u3092\u5165\u624b\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u30bf\u30a4\u30c8\u30eb\u3092\u8aad\u3093\u3060\u3068\u304d\u3068\u540c\u3058\u8981\u9818\u3067\u5404\u6587\u3092\u90e8\u5206\u3054\u3068\u306b\u89e3\u4f53\u3057\u3001\u524d\u304b\u3089\u9806\u306b\u8aad\u307f\u307e\u3059\u3002\u7dba\u9e97\u306a\u65e5\u672c\u8a9e\u3067\u7406\u89e3\u3059\u308b\u3053\u3068\u306b\u3053\u3060\u308f\u3089\u305a\u3001\u540c\u6642\u901a\u8a33\u3059\u308b\u3088\u3046\u306a\u8981\u9818\u3067\u6642\u306b\u306f\u60c5\u5831\u3092\u7e70\u308a\u8fd4\u3057\u306a\u304c\u3089\u3001\u307e\u305f\u3001\u30ab\u30bf\u30ab\u30ca\u8a9e\u3082\u4ea4\u3048\u306a\u304c\u3089\u7406\u89e3\u3092\u9032\u3081\u308b\u306e\u304c\u3088\u3044\u3067\u3057\u3087\u3046\u3002<\/p>\n

    \u5192\u982d\u300c\u4e3b\u984c\u3084\u554f\u984c\u306e\u63d0\u793a\u300d\u306e1\u6587\u76ee\u304b\u3089\u78ba\u8a8d\u3057\u307e\u3059\u3002<\/p>\n

    1. \u4e3b\u984c\u3084\u554f\u984c\u306e\u63d0\u793a
    \n1\u6587\u76ee<\/p>\n<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    High purity of plasmid DNA (pDNA),<\/td>\n\u30d7\u30e9\u30b9\u30df\u30c9DNA\uff08pDNA\uff09\u306e\u9ad8\u7d14\u5ea6\u5316<\/td>\n<\/tr>\n
    particularly in supercoiled isoform (SC),<\/td>\n\u7279\u306b\u3001\u8d85\u87ba\u65cb\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u3067\u306e\u9ad8\u7d14\u5ea6\u5316\u306f<\/td>\n<\/tr>\n
    is used<\/td>\n\u7528\u9014\u306b<\/td>\n<\/tr>\n
    for various biopharmaceutical applications,<\/td>\n\u5404\u7a2e\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u304c\u3042\u308b<\/td>\n<\/tr>\n
    such as<\/td>\n\u4f8b\u306f<\/td>\n<\/tr>\n
    a transfecting agent<\/td>\n\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u8a66\u85ac<\/td>\n<\/tr>\n
    for production<\/td>\n\u7528\u9014\u306f\u751f\u6210<\/td>\n<\/tr>\n
    of gene therapy viral vectors,<\/td>\n\u907a\u4f1d\u5b50\u6cbb\u7642\u306e\u305f\u3081\u306e\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306e<\/td>\n<\/tr>\n
    for pDNA vaccines,<\/td>\n\u30d7\u30e9\u30b9\u30df\u30c9DNA\u30ef\u30af\u30c1\u30f3\u306e\u305f\u3081<\/td>\n<\/tr>\n
    or<\/td>\n\u307e\u305f\u306f<\/td>\n<\/tr>\n
    as a precursor for linearized form<\/td>\n\u76f4\u9396\u72b6\u306e\u524d\u99c6\u4f53\u3068\u3057\u3066\u306e<\/td>\n<\/tr>\n
    that serves as a template<\/td>\n\u305d\u306e\u524d\u99c6\u4f53\u306f\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u306a\u308b<\/td>\n<\/tr>\n
    for mRNA synthesis.<\/td>\nmRNA\u5408\u6210\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u306a\u308b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

    \n
    \n

    \u7528\u8a9e\uff08Wikipedia\u3088\u308a\uff09\uff1a\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc(\u82f1: viral vector)\u306f\u3001\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u306b\u304a\u3044\u3066\u907a\u4f1d\u7269\u8cea\u3092\u7d30\u80de\u306b\u9001\u9054\u3059\u308b\u305f\u3081\u306b\u4e00\u822c\u7684\u306b\u4f7f\u7528\u3055\u308c\u308b\u907a\u4f1d\u5b50\u306e\u904b\u3073\u5c4b\u3067\u3042\u308b\u30d9\u30af\u30bf\u30fc\u306e\u3046\u3061\u3001\u30a6\u30a4\u30eb\u30b9\u3092\u30d9\u30fc\u30b9\u3068\u3057\u305f\u3082\u306e\u3002\uff08\u82f1\u6587\u306eWikipedia\u306f\u3053\u3061\u3089\uff1ahttps:\/\/en.wikipedia.org\/wiki\/Viral_vector\uff09<\/p>\n<\/div>\n

     <\/p>\n

    1\u3064\u306e\u6587\u7ae0\u3092\u89e3\u4f53\u3057\u3066\u524d\u304b\u3089\u9806\u306b\u8aad\u307f\u9032\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u305f\u3067\u3057\u3087\u3046\u304b\u3002\u82f1\u8a9e\u8ad6\u6587\u306e\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u306f\u3001\u5358\u8a9e\u6570\u306b\u5236\u9650\u304c\u3042\u308b\u305f\u3081\u3001\u540d\u8a5e\u53e5\u3092\u99c6\u4f7f\u3057\u3066\u60c5\u5831\u3092\u30b3\u30f3\u30d1\u30af\u30c8\u306b\u8a70\u3081\u8fbc\u3080\u6027\u8cea\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u4e00\u8aad\u3057\u305f\u3068\u304d\u306b\u9577\u304f\u3066\u8aad\u307f\u3065\u3089\u3044\u3068\u3044\u3046\u5370\u8c61\u3092\u53d7\u3051\u308b\u3053\u3068\u3082\u591a\u3044\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u304c\u3001\u5b9f\u969b\u306b\u306f\u3001\u3053\u306e\u3088\u3046\u306b\u90e8\u5206\u3054\u3068\u306b\u89e3\u4f53\u3057\u3066\u8aad\u3080\u3068\u3001\u96e3\u306a\u304f\u8aad\u307f\u9032\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u540d\u8a5e\u53e5\u3092\u99c6\u4f7f\u3057\u3066\u60c5\u5831\u304c\u52b9\u679c\u7684\u306b\u63d0\u793a\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n

    \u540c\u3058\u8981\u9818\u3067\u30012\u6587\u76ee\u3082\u8aad\u3093\u3067\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n

    2\u6587\u76ee<\/p>\n<\/div>\n\n\n\n\n\n\n\n\n\n\n
    In clinical manufacturing,<\/td>\n\u81e8\u5e8a\u88fd\u9020\u306b\u304a\u3044\u3066\u3001<\/td>\n<\/tr>\n
    pDNA is commonly extracted<\/td>\npDNA\uff08\u30d7\u30e9\u30b9\u30df\u30c9DNA\uff09\u306f\u901a\u5e38\u3001\u62bd\u51fa\u3059\u308b<\/td>\n<\/tr>\n
    from Escherichia coli cells with alkaline lysis<\/td>\n\u5927\u8178\u83cc\u306e\u30a2\u30eb\u30ab\u30ea\u6eb6\u89e3\u304b\u3089<\/td>\n<\/tr>\n
    followed by<\/td>\n\u7d9a\u3044\u3066<\/td>\n<\/tr>\n
    anion exchange chromatography<\/td>\n\u9670\u30a4\u30aa\u30f3\u4ea4\u63db\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc<\/td>\n<\/tr>\n
    or<\/td>\n\u307e\u305f\u306f<\/td>\n<\/tr>\n
    tangential flow filtration<\/td>\n\u30bf\u30f3\u30b8\u30a7\u30f3\u30b7\u30e3\u30eb\u30d5\u30ed\u30fc\u308d\u904e\u3092\u884c\u3046<\/td>\n<\/tr>\n
    as a capture step for pDNA.<\/td>\npDNA\u3092\u6355\u7372\u3059\u308b\u30b9\u30c6\u30c3\u30d7\u3068\u3057\u3066\u884c\u3046<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

    \u8aad\u307f\u9032\u3081\u308b\u969b\u3001\u6c17\u306b\u306a\u308b\u7528\u8a9e\u304c\u3042\u308c\u3070\u3001Wikipedia\u3067\u8abf\u3079\u308b\u3053\u3068\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002\u4f8b\u3048\u3070Alkaline lysis\uff08\u30a2\u30eb\u30ab\u30ea\u6eb6\u89e3\uff09https:\/\/en.wikipedia.org\/wiki\/Alkaline_lysis\u3084tangential flow filtration\uff08\u30bf\u30f3\u30b8\u30a7\u30f3\u30b7\u30e3\u30eb\u30d5\u30ed\u30fc\u308d\u904e\uff09https:\/\/en.wikipedia.org\/wiki\/Cross-flow_filtration\u3092\u8abf\u3079\u308b\u306e\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002<\/p>\n

    \u300c\u30a2\u30eb\u30ab\u30ea\u6eb6\u89e3\u300d\u306eWikipedia\u306b\u3088\u308b\u8aac\u660e\u306f\u6b21\u306e\u3068\u304a\u308a\u3067\u3059\u3002<\/p>\n

    \n
    \n

    Alkaline lysis or alkaline extraction is a method used in molecular biology to isolate plasmid DNA from bacteria.
    \n\u30a2\u30eb\u30ab\u30ea\u6eb6\u89e3\u3068\u306f\u3001\u30a2\u30eb\u30ab\u30ea\u62bd\u51fa\u3068\u3082\u547c\u3070\u308c\u3001\u5206\u5b50\u751f\u7269\u5b66\u306b\u304a\u3044\u3066\u30d0\u30af\u30c6\u30ea\u30a2\u304b\u3089\u30d7\u30e9\u30b9\u30df\u30c9DNA\u3092\u5358\u96e2\u3059\u308b\u305f\u3081\u306b\u7528\u3044\u3089\u308c\u308b\u65b9\u6cd5\u3002<\/p>\n<\/div>\n

     <\/p>\n

    Wikipedia\u3092\u8aad\u3080\u969b\u306b\u306f\u3001\u6280\u8853\u5185\u5bb9\u3092\u7406\u89e3\u3057\u3066\u5b66\u3076\u3068\u3068\u3082\u306b\u3001\u82f1\u5358\u8a9e\u3084\u82f1\u8a9e\u8868\u73fe\u306b\u3082\u3042\u308f\u305b\u3066\u7740\u76ee\u3059\u308b\u3068\u3001\u3088\u308a\u52b9\u679c\u7684\u3067\u3059\u3002\u3053\u3053\u3067\u306f\u3001isolate\uff08\u5358\u96e2\u3059\u308b\uff09\u3068\u3044\u3046\u52d5\u8a5e\u304c\u767b\u5834\u3057\u307e\u3057\u305f\u3002molecular biology\uff08\u5206\u5b50\u751f\u7269\u5b66\uff09\u3067\u306f\u767b\u5834\u983b\u5ea6\u306e\u9ad8\u3044\u52d5\u8a5e\u3067\u3059\u3002<\/p>\n

    \u3055\u3066\u3001\u6b21\u306e\u90e8\u5206\u3078\u9032\u307f\u3001\u540c\u3058\u8981\u9818\u3067\u5185\u5bb9\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002<\/p>\n

    2. \u5b9f\u969b\u306b\u4f55\u3092\u884c\u3063\u305f\u304b\u306e\u8aac\u660e
    \n5\u6587\u76ee<\/p>\n<\/div>\n\n\n\n\n\n\n
    We developed<\/td>\n\u4eca\u56de\u6211\u3005\u304c\u958b\u767a\u3057\u305f\u306e\u306f<\/td>\n<\/tr>\n
    HIC-based polishing purification methodology<\/td>\nHIC\u3092\u4f7f\u3063\u305f\u7814\u78e8\u7cbe\u88fd\u65b9\u6cd5\u3067<\/td>\n<\/tr>\n
    that is highly selective<\/td>\n\u305d\u308c\u306f\u9078\u629e\u6027\u304c\u9ad8\u304f<\/td>\n<\/tr>\n
    for enrichment of SC pDNA.<\/td>\nSC\uff08\u8d85\u87ba\u65cb\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\uff09\u306epDNA\uff08\u30d7\u30e9\u30b9\u30df\u30c9DNA\uff09\u306e\u6fc3\u7e2e\u304c\u53ef\u80fd\u306b\u306a\u308b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n

     <\/p>\n

    HIC\u3084SC\u3001pDNA\u3068\u3044\u3063\u305f\u7565\u8a9e\u306f\u521d\u51fa\u7b87\u6240\u3067\u5b9a\u7fa9\u3055\u308c\u308b\u306e\u304c\u901a\u4f8b\u3067\u3059\u306e\u3067\u3001\u300c\u4f55\u306e\u7565\u8a9e\u3060\u3063\u305f\u304b\u300d\u3001\u308f\u304b\u3089\u306a\u304f\u306a\u3063\u305f\u5834\u5408\u306b\u306f\u3001\u521d\u51fa\u7b87\u6240\u306b\u623b\u3063\u3066\u78ba\u8a8d\u3057\u307e\u3059\u3002HIC\u306fHydrophobic interaction chromatography\uff08\u758e\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\uff09\u3001SC\u306fsupercoiled isoform\uff08\u8d85\u87ba\u65cb\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\uff09\u3001pDNA\u306fplasmid DNA\uff08\u30d7\u30e9\u30b9\u30df\u30c9DNA\uff09\u3068\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n

    \u6b21\u306b\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u6700\u5f8c\u306e\u90e8\u5206\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002<\/p>\n

    3. \u4e3b\u8981\u306a\u7d50\u679c\u3084\u793a\u5506\u306e\u63d0\u793a
    \n9\u6587\u76ee<\/p>\n\n\n\n\n\n\n\n\n\n
    We show<\/td>\n\u308f\u304b\u3063\u305f\u3053\u3068\u306f<\/td>\n<\/tr>\n
    good scalability<\/td>\n\u62e1\u5f35\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068<\/td>\n<\/tr>\n
    to at least 300 mg of >95% SC pDNA,<\/td>\n95\uff05\u3092\u8d8a\u3048\u308b\u8d85\u87ba\u65cb\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u30d7\u30e9\u30b9\u30df\u30c9DNA\u306e\u5c11\u306a\u304f\u3068\u3082300mg\u307e\u3067\u62e1\u5f35\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068<\/td>\n<\/tr>\n
    thus paving the way to<\/td>\n\u305d\u306e\u305f\u3081\u3001\u9053\u304c\u958b\u3044\u305f<\/td>\n<\/tr>\n
    increase the quality of genomic medicines<\/td>\n\u30b2\u30ce\u30e0\u533b\u85ac\u54c1\u306e\u54c1\u8cea\u5411\u4e0a\u3078\u306e<\/td>\n<\/tr>\n
    that utilize pDNA<\/td>\npDNA\uff08\u30d7\u30e9\u30b9\u30df\u30c9DNA\uff09\u3092\u5229\u7528\u3059\u308b\u533b\u85ac\u54c1<\/td>\n<\/tr>\n
    as a key raw material.<\/td>\n\u4e3b\u8981\u539f\u6599\u3068\u3057\u3066\u5229\u7528\u3059\u308b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

    \u7565\u8a9e\u306b\u99b4\u67d3\u307f\u304c\u306a\u3051\u308c\u3070\u3001\u6700\u5f8c\u307e\u3067\u7565\u8a9e\u306e\u610f\u5473\u3092\u78ba\u8a8d\u3057\u306a\u304c\u3089\u8aad\u3080\u3068\u3088\u3044\u3067\u3057\u3087\u3046\u3002\u5206\u91ce\u306b\u7cbe\u901a\u3057\u3066\u3044\u308b\u5834\u5408\u306b\u306f\u3001\u7565\u8a9e\u3092\u305d\u306e\u307e\u307e\u4f7f\u3063\u3066\u8aad\u307f\u9032\u3081\u307e\u3057\u3087\u3046\u3002<\/p>\n

    \u3053\u306e\u3088\u3046\u306b\u3001\u82f1\u8a9e\u8ad6\u6587\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u3092\u6e96\u5099\u3057\u3001\u307e\u305a\u306f\u5168\u4f53\u3092\u628a\u63e1\u3059\u308b\u305f\u3081\u306b\u5927\u307e\u304b\u306b\u4e3b\u8a9e\u3068\u52d5\u8a5e\u3092\u78ba\u8a8d\u3057\u3001\u7d9a\u3044\u3066\u8208\u5473\u306e\u3042\u308b\u7b87\u6240\u3092\u533a\u5207\u308a\u306a\u304c\u3089\u8a73\u7d30\u306b\u8aad\u307f\u9032\u3081\u307e\u3059\u3002\u307e\u308b\u3067\u540c\u6642\u901a\u8a33\u3092\u3059\u308b\u3088\u3046\u306b\u7d30\u304b\u304f\u533a\u5207\u308a\u306a\u304c\u3089\u8aad\u307f\u9032\u3081\u308b\u3053\u3068\u3067\u3001\u82f1\u5358\u8a9e\u3060\u3051\u3067\u610f\u5473\u3092\u7406\u89e3\u3057\u3066\u3057\u307e\u3046\u3053\u3068\u306a\u304f\u3001\u3064\u307e\u308a\u81ea\u5206\u81ea\u8eab\u306e\u77e5\u8b58\u3067\u5185\u5bb9\u3092\u88dc\u3046\u3053\u3068\u306b\u3088\u3063\u3066\u8aad\u307f\u9055\u3048\u3066\u3057\u307e\u3046\u3053\u3068\u306a\u304f\u3001\u69cb\u6587\u3092\u6b63\u3057\u304f\u89e3\u8aad\u3057\u306a\u304c\u3089\u3001\u3057\u304b\u3082\u5e73\u6613\u304b\u3064\u77ed\u6642\u9593\u3067\u8aad\u307f\u9032\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u306e\u3067\u3059\u3002<\/p>\n

    \u3055\u3066\u3001\u3053\u306e\u3088\u3046\u306a\u8981\u9818\u3067\u82f1\u8a9e\u8ad6\u6587\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u30921\u65e51\u4ef6\u8aad\u307f\u9032\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u308c\u3070\u3001\u82f1\u8a9e\u8ad6\u6587\u30a2\u30d6\u30b9\u30c8\u30e9\u30af\u30c8\u306e\u8868\u73fe\u306e\u4e2d\u3067\u3001\u304a\u624b\u672c\u3068\u3057\u3066\u771f\u4f3c\u3092\u3057\u305f\u3044\u5b9a\u756a\u306e\u8868\u73fe\u304c\u3042\u308b\u3053\u3068\u306b\u3082\u6c17\u4ed8\u304f\u3067\u3057\u3087\u3046\u3002\u8a73\u7d30\u306b\u8aad\u307f\u9032\u3081\u308b\u3053\u3068\u3067\u3001\u305d\u306e\u3088\u3046\u306a\u5b9a\u756a\u8868\u73fe\u306b\u6c17\u4ed8\u304f\u53ef\u80fd\u6027\u304c\u9ad8\u307e\u308a\u3001\u771f\u4f3c\u3092\u3057\u305f\u3044\u8868\u73fe\u304c\u898b\u3064\u304b\u308c\u3070\u3001\u305d\u308c\u3092\u4f7f\u3063\u3066\u81ea\u8eab\u306e\u82f1\u8a9e\u8ad6\u6587\u3092\u57f7\u7b46\u3057\u3066\u3044\u304f\u3053\u3068\u304c\u5bb9\u6613\u306b\u306a\u308a\u307e\u3059\u3002\u6628\u4eca\u3001\u7cbe\u5ea6\u304c\u9ad8\u307e\u3063\u305f\u3068\u8a00\u308f\u308c\u308b\u6a5f\u68b0\u7ffb\u8a33\u3084\u751f\u6210AI\u30c4\u30fc\u30eb\u3092\u4f7f\u3046\u5834\u5408\u3067\u3042\u3063\u3066\u3082\u3001\u81ea\u5206\u306e\u529b\u3067\u8aad\u3081\u308b\u3001\u81ea\u5206\u306e\u529b\u3067\u66f8\u3051\u308b\u3001\u3068\u3044\u3046\u30b9\u30ad\u30eb\u3092\u8eab\u306b\u3064\u3051\u308b\u3053\u3068\u306f\u3001\u30c4\u30fc\u30eb\u306e\u4e0a\u624b\u306a\u4f7f\u3044\u3053\u306a\u3057\u306e\u305f\u3081\u306b\u91cd\u8981\u3067\u3059\u3002<\/p>\n

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